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. 2023 May 11;12:e85041. doi: 10.7554/eLife.85041

Figure 6. Tracing connections between the periphery and the CNS with retro-Tango.

(A) Expression of the retro-Tango ligand in SAG neurons reveals (B) SPSNs (asterisk) as presynaptic partners. (C) When retro-Tango is initiated from Or67d-expressing ORNs, OPNs (arrow) and LNs (arrowhead) are revealed as their presynaptic partners(134±17 neurons in 5 brains). 15do females heterozygous for the tdTomato reporter (A) and males (B) were analyzed. Postsynaptic GFP (cyan), presynaptic mtdTomato (magenta) and neuropil (A, C), or phalloidin (B) (grey). Scale bars, 50 μm.

Figure 6.

Figure 6—figure supplement 1. Initiating trans-Tango from the Or67d-expressing ORNs.

Figure 6—figure supplement 1.

Initiating trans-Tango from the Or67d-expressing ORNs results in strong postsynaptic signal in OPNs and LNs (102±17 neurons in 5 brains). Note the labeling in the mediolateral antennal lobe tract (arrow). 20do males were analyzed for both panels. Presynaptic GFP (cyan), postsynaptic mtdTomato (magenta) and neuropil (grey). Scale bars, 50 μm.