Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic Reagent (D. melanogaster) |
GF-split-Gal4 | von Reyn et al., 2014 | RRID: BDSC#79602 | Flybase symbols: P{R17A04-p65.AD} P{R68A06-GAL4.DBD} |
| Genetic Reagent (D. melanogaster) |
Or67dGal4 | Kurtovic et al., 2007 | FlyBase: FBti0168583 | Flybase symbol: TI{GAL4}Or67dGAL4-1 |
| Genetic Reagent (D. melanogaster) |
ss00090-Gal4 | Wolff and Rubin, 2018 | RRID: BDSC#75849 | Flybase symbols: P{R15C03-GAL4.DBD} P{R19G02-p65.AD} |
| Genetic Reagent (D. melanogaster) |
SAG-split-Gal4 | Feng et al., 2014 | RRID: BDSC#66875 | Flybase symbols: P{VT007068-GAL4.DBD} P{VT050405-p65.AD} |
| Genetic Reagent (D. melanogaster) |
aDN-split-Gal4 | Nojima et al., 2021 | FlyBase:FBal0243326 FlyBase: FBal0325783 |
Flybase symbols: P{dVP16AD}VGlutOK371-dVP16AD TI{GAL4(DBD)::Zip-}dsxGAL4-DBD |
| Genetic Reagent (D. melanogaster) |
QUAS-nls-DsRed | Snell et al., 2022 | RRID: BDSC#95315 | Isolated from BDSC#95315 Flybase symbol: P{5xQUAS-nlsDsRedT4}su(Hw)attP8 |
| Genetic Reagent (D. melanogaster) |
QUAS-mtdTomato(3xHA) | This study | Will be deposited to Bloomington Drosophila Stock Center | |
| Genetic Reagent (D. melanogaster) |
retro-Tango(panneuronal) | This study | Will be deposited to Bloomington Drosophila Stock Center | |
| Genetic Reagent (D. melanogaster) |
retro-Tango(ligand) | This study | Will be deposited to Bloomington Drosophila Stock Center | |
| Genetic Reagent (D. melanogaster) |
MB247-Gal4 | Aso et al., 2009 | RRID: BDSC#50742 | Flybase symbol: P{Mef2-GAL4.247} |
| Genetic Reagent (D. melanogaster) |
UAS-syt::GFP | Zhang et al., 2002 | RRID: BDSC#6924 | Flybase symbol: P{UAS-syt.eGFP} |
| Genetic Reagent (D. melanogaster) |
Reporters +trans-Tango | Talay et al., 2017 | RRID: BDSC#77124 | Flybase symbols: P{trans-Tango} P{UAS-myrGFP.QUAS-mtdTomato-3xHA} |
| Antibody | α-GFP (chicken polyclonal) | Gift from Susan Brenner-Morton (Columbia University) | IHC (1:10000) | |
| Antibody | α-RFP (guinea pig polyclonal) | Gift from Susan Brenner-Morton (Columbia University) | IHC (1:10000) | |
| Antibody | α-Brp (mouse monoclonal) | DSHB | RRID: AB_2314866 | IHC (1:20) |
| Antibody | α-chicken 488 (donkey polyclonal) | Jackson ImmunoResearch # 703-546-155 |
RRID: AB_2340376 | IHC (1:1000) |
| Antibody | α-guinea pig 555 (donkey polyclonal) | Jackson ImmunoResearch # 706-165-148 |
RRID: AB_2340460 | IHC (1:1000) |
| Antibody | α-mouse 647 (donkey polyclonal) | Thermo Fisher Scientific #A-31571 | RRID: AB_162542 | IHC (1:1000) |
| Chemical compound, drug | Phalloidin 647 | Thermo Fisher Scientific | Catalog number: A22287 | (1:500) |