Fig. 10.

Suppression of APP processing induces clustering of APP degradation products, TH, HS-anMan, and SYNfil in differentiating cells (NPC-iN). Representative immunofluorescence images of cells that were maintained in A, C, E) complete (+0.1 μM Asc) differentiation medium without further additions (−) or in B, D, F) complete medium containing 100 nM LY2811376 (+β-inhibitor). Staining was performed with mAb 82E1 (for the N-terminal of β-CTF/Αβ, green), mAb AM (for HS-anMan, green), mAb SYNfil (green), pAb anti-TH (red), and DAPI (for nuclei, blue). Exposure time was the same in all cases. Bar, 20 μm.