Fig. 6.

Inhibition of β-secretase suppresses IL-6-induced formation of β-CTF, HS-anMan, and SYNfil. Representative immunofluorescence images of cells that were grown to confluence in A, D, G) regular medium or in B, E, H) medium containing 100 ng/mL IL-6 or in C, F, I) medium containing 100 ng/mL IL-6 and 100 nM β-inhibitor (LY2811376). Staining was performed with mAb 82E1 (for the N-terminal of β-CTF/Αβ, green), mAb AM (for HS-anMan, green), mAb SYNfil (green), pAb A8717 (for the C-terminal of APP/β-CTF, red), pAb SYN (red), and DAPI (for nuclei, blue). Exposure time was the same in all cases. Bar, 20 μm.