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. 2023 Feb 15;33(4):325–341. doi: 10.1093/glycob/cwad013

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 9 — Suppression of HS-anMan release from GPC1 during differentiation of NPC to NPC-iN induces coclustering of SYNfil and TH. Representative immunofluorescence images of cells that were maintained in A, D) complete (+0.1 μM Asc) differentiation medium without further additions, or in B, E) complete medium containing 100 μM SMTC, or in C, F) complete medium containing 100 μM SMTC, 150 μM allopurinol, and 1 μM SB203580. Staining was performed with mAb AM (for HS-anMan, green), mAb SYNfil (green), pAb anti-TH (red), and DAPI (for nuclei, blue). Exposure time was the same in all cases. Bar, 20 μm.