FIGURE 1.

HCC is aggravated in FxrKO mice on Tlr5 deletion. (A–I) Male WT (n = 5), Tlr5KO (n = 5), FxrKO (n = 16), and DKO (n = 18) mice were aged for 16 months, and liver cancer parameters were analyzed. (A) Gross liver, (B) tumor number, (C) tumor size (diameter, mm), (D) H&E staining at scale bars of i. 2 mm and ii. 50 µm, (E) liver histology score, (F) cytokeratin 19 immunohistochemistry (CK19; scale bar, 50 μm), (G) serum α-fetoprotein (AFP), and quantitative RT-PCR of hepatic (H) Gpc3 and (I) Myc. 36b4 was used as internal control for RT-qPCR. Results are expressed as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Abbreviations: DKO, double knockout; RT-PCR, real-time PCR; WT, wild type.