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. 2023 May 23;7(6):e0166. doi: 10.1097/HC9.0000000000000166

FIGURE 4.

FIGURE 4

Gut microbiota for the DKO mice is enriched in HCC-associated Proteobacteria. (A–C) Male WT (n = 5 or 15), Tlr5KO (n = 5), FxrKO (n = 16), and DKO (n = 18) mice were aged for 16 months, fecal samples were snap frozen and bacterial DNA was extracted for RT-qPCR and 16S microbiota analysis. (A) Total bacterial load (copy number), (B) β-diversity through Bray-Curtis, and (C) Linear discriminant analysis Effect Size (LEfSe) comparing FxrKO and DKO groups. Results are expressed as means ± SEM. *p < 0.05. Raw data are deposited into the public database GenBank (BioProject ID: PRJNA941305, BioSample accessions: SAMN33601343, SAMN33601344, SAMN33601345, SAMN33601346). Abbreviations: DKO, double knockout; LDA, linear discriminant analysis; WT, wild type.