Figure 4.
CanB19G provides a fitness advantage in the context of gyrase mutations. (A) Competition between ciprofloxacin resistant isogenic CanB strains and susceptible parental strains (N=3, representative of two independent experiments, error bars represent SEM). Significance (from left to right, p = 0.00063, p = 0.0019) by unpaired two-sided t-test. (B) Growth curves of CanB isogenic FA19 strains with ciprofloxacin resistance-determining gyrA alleles (N=12, data from two independent experiments, error bars represent SEM). Significance (from left to right, p = 0.018, 0.048, 5.0e-5, 9.7e-5, 0.00041, 0.00019) by unpaired two- sided t-test. (C) Volcano plot of RNA-seq showing transcript levels compared between FA19 CanBG19E and FA19 CanBG19E GyrA91F by DeSeq2. Specific transcripts of interest are highlighted in color. (D) Competition between FA19 CanBG19E and FA19 CanBG19E GyrAS91F after 16 hours along with media supplementation in liquid GCP medium (N=6, representative of two independent experiments, error bars represent SEM). Significance (p = 0.99, p = 2.2e-6, p = 3.3e-9) by one-way ANOVA with Dunnett’s multiple comparisons test. *p<0.05, **p<0.01, ***p<0.001