Extended Data Fig. 5.

Loss of CanB does not appear to confer a loss of natural competence. (A) Plating efficiency in the presence and absence of CO₂ for a knockout of CanB in the N. gonorrhoeae lab strain FA1090 (N=6, from two independent experiments, error bars represent SEM). Significance (p=0.0006) by two-sided Mann-Whitney U. (B) Natural competence of strains of N. gonorrhoeae as measured by uptake of a nalidixic acid resistance-conferring integrating plasmid (N=3, representative of two independent experiments, error bars represent SEM). (C and D) As in (B), natural competence as measured by (C) uptake of Kan^R-conferring plasmid DR1 and (D) a PCR product containing the allele encoding for GyrA^91F/95G (N=6, representative of two independent experiments, error bars represent SEM). *p<0.05, **p<0.01, ***p<0.001