Table 1.
Feed formulation and chemical composition of diets for largemouth bass (g/kg, air dry basis).
| Item | Groups |
|||
|---|---|---|---|---|
| Control | BA150 | BA300 | BA450 | |
| Ingredients | ||||
| Cassava starch | 100.00 | 100.00 | 100.00 | 100.00 |
| Soybean meal | 120.00 | 120.00 | 120.00 | 120.00 |
| Groundnut meal | 100.00 | 100.00 | 100.00 | 100.00 |
| Fish meal | 400.00 | 400.00 | 400.00 | 400.00 |
| Chicken meal | 150.00 | 150.00 | 150.00 | 150.00 |
| Bile acid1 | 0.00 | 0.15 | 0.30 | 0.45 |
| Bentonite | 6.00 | 5.85 | 5.70 | 5.55 |
| Lys-HCl | 8.00 | 8.00 | 8.00 | 8.00 |
| Methionine | 2.00 | 2.00 | 2.00 | 2.00 |
| Choline chloride | 3.00 | 3.00 | 3.00 | 3.00 |
| Ca(H2PO4)2 | 10.00 | 10.00 | 10.00 | 10.00 |
| Soybean oil | 60.00 | 60.00 | 60.00 | 60.00 |
| Phosphatide oil | 20.00 | 20.00 | 20.00 | 20.00 |
| Vitamin C | 1.00 | 1.00 | 1.00 | 1.00 |
| Vitamin premix2 | 10.00 | 10.00 | 10.00 | 10.00 |
| Mineral premix3 | 10.00 | 10.00 | 10.00 | 10.00 |
| Total | 1000 | 1000 | 1000 | 1000 |
| Chemical composition | ||||
| Crude protein | 516.80 | 520.00 | 515.10 | 517.20 |
| Crude fat | 147.10 | 142.10 | 144.90 | 145.80 |
| Gross energy, MJ/kg | 18.72 | 18.72 | 18.72 | 18.72 |
Provided by Shandong Longchang Animal Health Care Co., Ltd., Dezhou, China, its composition was as follows: 8.00% hyocholic acid, 70.67% hyodeoxycholic acid, and 19.61% chenodeoxycholic acid.
Vitamin premix containing the following (g/kg vitamin premix): thiamine, 0.438; riboflavin, 0.632; pyridoxine⋅HCl, 0.908; D-pantothenic acid, 1.724; nicotinic acid, 4.583; biotin, 0.211; folic acid, 0.549; vitamin B12, 0.001; inositol, 21.053; menadione sodium bisulfite, 0.889; retinyl acetate, 0.677; cholecalciferol, 0.116; DL-α-tocopherol-acetate, 12.632.
Mineral premix containing the following (g/kg mineral premix): CoCl2⋅6H2O, 0.074; CuSO4⋅5H2O, 2.5; FeSO4⋅7H2O, 73.2; NaCl, 40.0; MgSO4⋅7H2O, 284.0; MnSO4⋅H2O, 6.50; KI, 0.68; Na2SeO3, 0.10; ZnSO4⋅7H2O, 131.93; Cellulose, 501.09.