Table 1.
Formulation and proximate composition of the experimental diets (% dry matter).
| Item | Diets (n-3/n-6 PUFA ratio) |
|||||
|---|---|---|---|---|---|---|
| Diet 1 (0.04) | Diet 2 (0.35) | Diet 3 (0.66) | Diet 4 (1.35) | Diet 5 (2.45) | Diet 6 (16.17) | |
| Ingredients | ||||||
| White fish meal | 25.00 | 25.00 | 25.00 | 25.00 | 25.00 | 25.00 |
| Soy protein concentrate | 27.97 | 27.97 | 27.97 | 27.97 | 27.97 | 27.97 |
| Wheat gluten meal | 12.00 | 12.00 | 12.00 | 12.00 | 12.00 | 12.00 |
| Corn starch | 18.00 | 18.00 | 18.00 | 18.00 | 18.00 | 18.00 |
| DHA + EPA-enriched oil1 | 0.00 | 3.00 | 4.50 | 6.00 | 7.20 | 9.00 |
| LA-enriched oil2 | 9.00 | 6.00 | 4.50 | 3.00 | 1.80 | 0.00 |
| Microcrystalline cellulose | 4.68 | 4.68 | 4.68 | 4.68 | 4.68 | 4.68 |
| Vitamin C | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| Premix3 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 |
| Choline chloride | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| CaH2PO4 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Proximate composition | ||||||
| Dry matter | 93.42 | 95.26 | 94.18 | 95.32 | 94.56 | 95.21 |
| Protein | 43.84 | 45.16 | 45.33 | 45.06 | 45.18 | 44.02 |
| Lipid | 10.30 | 10.65 | 10.22 | 10.39 | 10.44 | 10.19 |
| Ash | 9.75 | 9.77 | 9.65 | 9.89 | 9.86 | 9.64 |
PUFA = poly-unsaturated fatty acids; DHA = docosahexaenoic acid; EPA = eicosapentaenoic acid; LA = linoleic acid.
1
DHA + EPA-enriched oil: DHA content, 413.5 mg/g oil; EPA content, 424.3 mg/g oil.
2
LA-enriched oil: LA content, 853.2 mg/g oil.
3
The composition of premix was described in our previous study (Cai et al., 2020).