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Terminated transcripts are released from ternary complexes. After a 15-min transcription reaction, parallel transcription reactions were stopped immediately (lane 2) or chased for 5 min at 30°C with 1 mM GTP (lane 4), 1 mM GTP plus 1 mM CTP (lane 5), or an equivalent volume of transcription buffer (lane 3). Sizes are indicated in nucleotides.
