FIG. 5.

Terminated transcripts are polyadenylated. (A) Time course of transcription and polyadenylation. In vitro transcription reactions with Polh/CFS-T and purified RNA polymerase were stopped at the times indicated above the lanes. RNA transcripts were resolved on a 6% polyacrylamide–8 M urea gel. Lane 1, φX174/HinfI marker. Positions of the relevant transcripts are indicated in nucleotides on the right. (B) Sequences of cDNA clones. RNA 3′ RACE and cDNA sequencing were used to determine the sites of transcription termination. The sequence corresponds to the nontemplate strand of the globin cleavage/polyadenylation signal in Polh/CFS-T; the poly(A) signal is underlined. The viral RNA polymerase terminates at the end of two T-rich sequences in the globin sequence. Three clones contained two nontemplated T residues before the poly(A) tails.