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ATP hydrolysis is not required for transcription termination by baculovirus RNA polymerase. Purified RNA polymerase (0.2 pmol) was incubated with 0.2 pmol of Polh/CFS-T plasmid at 30°C for 60 min in standard transcription buffer containing 1 mM ATP (lane 2) or 1 mM ATPγs to replace ATP. Lane 1, φX174/HinfI marker. Sizes are indicated in nucleotides.
