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. Author manuscript; available in PMC: 2024 Jan 6.
Published in final edited form as: J Vis Exp. 2023 Jan 6;(191):10.3791/64448. doi: 10.3791/64448

Figure 2: Immunostaining of isolated endometrial epithelium and stromal cell populations.

Figure 2:

Immunofluorescence images show epithelial and stromal cell populations of the (A,B) epithelial cell and (C,D) stromal cell fractions following enzymatic and mechanical separation of the uterus. Cytokeratin 8 (an epithelial cell marker) is shown in red, vimentin (a stromal cell marker) is shown in green, and DAPI (a nuclear marker) is shown in blue. Scale bars = 100 μm (A,C), 20 μm (B,D). Abbreviations: CK8 = cytokeratin 8; VIM = vimentin; DAPI = 4′,6-diamidino-2-phenylindole.