Table 1:
Primer sequences and PCR conditions.
| Primers and PCR Conditions | ||
|---|---|---|
| Primer Sequence | Forward (5′-3′) | Reverse (5′-3′) |
| Lipocalin 2 (Lcn2) | GCAGGTGGTACGTTGTGGG | CTCTTGTAGCTCATAGATGGTGC |
| Lactoferrin (Ltf) | TGAGGCCCTTGGACTCTGT | ACCCACTTTTCTCATCTCGTTC |
| Progesterone (Pgr) | CCCACAGGAGTTTGTCAAGCTC | TAACTTCAGACATCATTTCCGG |
| Glyceraldehyde 3 phosphate dehydrogenase (Gapdh) | CAATGTGTCCGTCGTGGATCT | GCCTGCTTCACCACCTTCTT |
| PCR mixture contains: |
|---|
| 2X SYBR Green |
| 0.5μM Fwd Primer |
| 0.5μM Rev Primer |
| cDNA |
| RNAse/DNAse-Free H20 |
| Cycler Conditions: | |||
|---|---|---|---|
| Temperature | Time | Cycles | |
| Hold | 95 C | 10 min | 1 |
| Denature | 95 C | 15 sec | 40 |
| Extend | 60 C | 60 sec |