Table 2.

Primers used for real-time quantitative PCR.

Target gene	Primer sequence (5′–3′)	Amplicon length (bp)	Reference	Accession number
Reference genes
GAPDH	F: CAACCCCCAATGTCTCTGTT R: TCAGCAGCAGCCTTCACTAC	94	Gilani et al., 2018	NM_204305
TBP	F: GTCCACGGTGAATCTTGGTT R: GCGCAGTAGTACGTGGTTCTC	128	Gilani et al., 2018	NM_205103
β-actin	F: GAGAAATTGTGCGTGACATCA R: CCTGAACCTCTCATTGCCA	152	Liu et al., 2015	NM_205518
Steroid receptors
AR	F: AAGAAGCTGGGCAGTCTGAAR: AGCAGGTTGGAGAAGGAGTC	214	Li et al., 2020	NM_001040090
ERα	F: TATTGATGATCGGCTTAGTCTGGCR: CGAGCAGCAGTAGCCAGTAGCA	145	Liu et al., 2015	NM_205183.2
PR	F: ATCATCGTTCTATTCACTGT R: CTCGTTCTCATCTCATCAA	168	Liu et al., 2015	NM_205262.1
Satellite cell development genes
Pax7	F: AGTTCGATTAGCCGTGTGCT R: CTCTTCAAAGGCAGGTCTGG	185	Li et al., 2020	NM_205065
MyoD	F: ATGTCCCATACTGCCTCCAG R: GTCTTGGAGCTTGGCTGAAC	235	Li et al., 2020	NM_204214
MyoG	F: GGCTTTGGAGGAGAAGGACT R: CAGAGTGCTGCGTTTCAGAG	N.P	Cazzato et al., 2014	NM_204184
Adipocyte differentiation genes
PPARγ	F: CCACTGCAGGAACAGAACAA R: CTCCCGTGTCATGAATCCTT	249	Piestun et al., 2017	NM_001001460.1
CEBP/β	F: GCCGCCCGCCTTTAAA R: CCAAACAGTCCGCCTCGTAA	N.P	Zhang et al., 2015	NM_205253.2

N.P, not provided.