Table 1.

Summary of the stability of 417 lipids from 13 different classes in whole blood after collection

	Cooled at once 4°C Time [h]						Room temp. 21°C Time [h]						Summer time conditions 30°C Time [h]
	0.5	1	1.5	2	4	24	0.5	1	1.5	2	4	24	0.5	1	1.5	2	4	24
Lipid class
FA (n = 40a)						10		1	2	2	10	25		2	4	5	14	29
LPC (n = 30)						5		5	11	12	22	27	12	19	23	25	27	29
LPE (n = 8)								1	1		3	6	1	3	3	3	5	7
PC (n = 80)												9					2	21
PE (n = 26)																		13
PG (n = 2)																		1
PI (n = 17)
Cer (n = 21)																		2
HexCer (n = 7)
SM (n = 34)
SE (n = 9)												6					1	7
DG (n = 11)						1				1	3	11		2	3	6	11	11
TG (n = 132)						3					3	8	1				1	9
Sum (%)						19		7	14	15	41	92	14	26	33	39	61	129
						(4.6%)		(1.7%)	(3.4%)	(3.6%)	(9.8%)	(22.1%)	(3.4%)	(6.2%)	(7.9%)	(9.4%)	(14.6%)	(30.9%)

The numbers in each column correspond to lipids defined as unstable according to the following criteria: P < 0.05 and FDR <0.05 and >10% change in signal intensities in comparison to samples prepared at once (0.5–1.5 h, n = 27 per time point; 2–24 h, n = 56 per time point). Details of each significantly altered lipid species after 24 h exposure (e.g. significance level, percentage of alteration) are provided in supplementals Table S2 and S3.

Cer, ceramide; DG, diacylglycerol, FA, free fatty acid; HexCer, hexosylceramide; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; SE, cholesterol ester; SM, sphingomyelin; TG, triacylglycerol.

^atotal number of covered lipid species in the lipid class.