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. 2023 Apr 14;299(6):104718. doi: 10.1016/j.jbc.2023.104718

Figure 5.

Figure 5

Negative-staining EM of VPS33B–VPS16B complex.A, representative micrograph of negative-stained glutaraldehyde-crosslinked purified VPS33B–VPS16B-3xFLAG complex; circles indicate representative particles used for 2D class averaging. Micrographs collected at 25,000× magnification with image pixel size of 2.61 Å; bar represents 200 nm. B, 2D class averages generated from 407 particles reveal an elongated two-lobed complex structure; bar represents 10 nm. VPS, vacuolar protein sorting protein.