FIG. 2.
Protein and genomic RNA composition of wild-type (wt) and vif mutant (vifΔ) virions from H9 cells. Protein profiles of virions derived from chronically infected H9 cells expressing wild-type and mutant constructs were evaluated by SDS-PAGE, and immunoblots were stained with HIV-1-positive patient serum (A), antiserum against p7NC (B), antiserum against p17MA (C), antiserum against p6gag (D), a monoclonal antibody against RT (E), or antiserum against IN (F). (G) Virion-associated genomic RNA was hybridized with a probe directed against the 5′ LTR and gag coding regions and visualized by autoradiography.