FIG. 3.

Intravirion nucleic acid profiles from Jurkat and H9 cells expressing wild-type (wt) or vif mutant (vifΔ) constructs. Nucleic acids were extracted from Jurkat cell-derived virions. (A and B) tRNA₃^Lys was analyzed by Northern blot hybridization (A), and (B) viral genomic RNA content was analyzed by slot blot analysis (B) with two separate dilutions (3 and 1 μl) of each sample. (C) Natural virion-associated RT products were detected by PCR with specific primers, followed by hybridization with a probe directed against the 5′LTR and gag coding region. The primers are described in Materials and Methods. Control standards were amplified with the indicated primers and the HXB2 construct as the template in serial 10-fold dilutions. Similar analysis was performed for (D) tRNA₃^Lys (D), virion-associated genomic RNA (E), and natural intravirion RT products (F) from virions derived from H9 cells expressing wild-type or vif mutant constructs.