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. 2023 May 24;14(5):340. doi: 10.1038/s41419-023-05859-0

Fig. 7. NAC inhibited ferroptosis by enhancing antioxidant capacity.

Fig. 7

A Representative images of H&E staining of ovarian micromorphology of mice after treatment with Cis or Cis + NAC. Scale bars = 200 μm. n = 5 (independent experiments). B, C ROS assay to evaluate the intracellular ROS levels of SVOG and KGN cells after Cis (25 μg/ml) or Cis +NAC (5 mM) treatments for 24 h. D, E The protein levels of GPX4, Nrf-2, and HO-1 were examined using Western blot analysis in SVOG (D) and KGN (E) cells treated with Cis (25 μg/ml) or Cis +NAC (5 mM) for 24 h. F, G Quantitative analysis of the targeted protein expressions. n = 3 (independent experiments). H, I The ratio of Intracellular GSH/GSSG was assayed after Cis (25 μg/ml) or Cis +NAC (5 mM) treatment for 24 h in SVOG (H) and KGN (I) cells. n = 5 (independent experiments). J, K Intracellular MDA were assayed after Cis (25 μg/ml) or Cis +NAC (5 mM) treatment for 24 h in SVOG (J) and KGN (K) cells. n = 5 (independent experiments). L, M Representative images of BODIPY staining indicated lipid ROS level after Cis (25 μg/ml) or Cis + NAC (5 mM) treatments (red for reduction, green for oxidization) in SVOG (L) and KGN (M) cells. Scale bar = 10 μm. n = 3 (independent experiments). *p < 0.05, **p < 0.01, ***p < 0.001. NS: not significant.