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. 2023 May 24;14:2985. doi: 10.1038/s41467-023-38426-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Dot plot showing the expression levels of cell type marker genes in 4 cell types. b t-SNE plot showing subclusters of B cells (coloured by cell type). c Heatmap plot showing subclusters of B cells. d Functional signalling pathway enrichment plot of MALT-B cells. MALT-B cells were mainly related to immune response, B-cell activation, and participation in complement activation. e Functional signalling pathway enrichment plot of follicular B cells. Follicular B cells were related to immune system and regulation of B cells. f Box plot of tumour-infiltrating follicular B and MALT-B cells in M/PDA and GSRC. Compared with M/PDA, tumour-infiltrating follicular B cells in GSRC were higher, the difference was not statistically significant (t-test, p = 0.19); Similarly, there is no difference in tumour-infiltrating MALT-B cells between M/PDA and GSRC (t-test, p = 1). n = 10 (include 3 samples in MDA and PDA groups, respectively; and 4 samples in GSRC group). Data are presented as mean values ± SEM. In the box plot, the black dots represent outliers, the error bars represent SEM, the box midpoints represent means and the boxes represent inter-quartile positions. g Pod plot of tumour-infiltrating follicular B and MALT-B cells in M/PDA and GSRC by IHC score (n = 30). Compared with that of M/PDA, the IHC score of tumour-infiltrating follicular B cells (expressing CD74) in GSRC was higher, and the difference was statistically significant (Wilcoxon rank-sum test, p = 0.012); The IHC score of tumour-infiltrating MALT-B cells (expressing JCHAIN) in GSRC was higher, but the difference was not statistically significant (Wilcoxon rank-sum test, p = 0.154). p values were calculated using Wilcoxon rank-sum test. h Pseudochronological analysis of subclusters of B cells. Starting from marker point 1, B cells were divided into two branches: one consisting of follicular B cells and memory B cells; the other consisting of MALT-B cells and plasma cells. In d, e and f, the statistical strategy were two-sided Student’s t-test. p values < 0.05 were considered to indicate significance: *p < 0.05; ns no significance. Source data are provided as a Source Data file.