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. 2023 May 24;13:8427. doi: 10.1038/s41598-023-35587-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ALA on apoptosis in heat stressed-porcine parthenotes. (A) Representative images of caspase3 after HT exposure in presence of ALA in porcine parthenotes. Red, caspase3; Blue, DNA. (B) Relative fluorescence intensity of caspase3 after HT exposure with ALA addition. (C) Western blotting of Bcl-xL protein expression after HT exposure with ALA treatment. Original blots are presented in Supplementary Fig. 1B′,C. (D) Relative band intensity analysis for Bcl-xL/GAPDH after HT exposure with ALA supplementation. *p < 0.05; **p < 0.01.