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. 2023 May 24;14(5):341. doi: 10.1038/s41419-023-05862-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B Representative micrographs of EdU positivity in cells transfected with Control and PAXIP1-AS1 (A) or Scr siRNA and PAXIP1-AS1 siRNAp (B). Red fluorescence represents EdU-positive cells and blue fluorescence from the Hoechst 33342 stain represents total cells. C, D Representative images of crystal violet stained cell colonies formed by the indicated GC cell, 14 d after inoculation. E Gross appearance and external whole-body fluorescence images of subcutaneous tumours from mice administered subcutaneous injection of MKN-74/RFP-Control and MKN-74/RFP-PAXIP1-AS1 cells. F The cell proliferation rate of PAXIP1-AS1 overexpressing tumours was significantly lower than that of Control, as observed by immunohistochemistry (IHC) assay with Ki-67. G–J Migration (G and I) and invasion (H and J) assays were conducted using GC cells transfected with PAXIP1-AS1 and Control (G and H) or PAXIP1-AS1 siRNAp and Scr siRNA (I and J). K, L The wound healing assay was used to detect GC cell motility following transfection with PAXIP1-AS1 and Control (K) or PAXIP1-AS1 siRNAp and Scr siRNA (L). M Lung samples were obtained from mice that received intravenous tail injections of MKN-74/PAXIP1-AS1 and Control cells, respectively (n = 3). N Representative images of livers from intrasplenic liver metastasis model constructed with MKN-74/PAXIP1-AS1 and control cells (n = 3). O The number of hepatic metastatic tumours of the control and PAXIP1-AS1 groups. Scale bars, 50 μm in A & B and 100 μm in F. For quantification, see Supplementary Figs. 3 & 4.