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. 2023 May 24;14(5):341. doi: 10.1038/s41419-023-05862-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The efficiency of HOXD9 plasmid transfection was detected by western blotting assay. B The morphology of GC cells was observed under phase-contrast microscopy. C Transfected AGS and MKN-74 cells were stained with rhodamine-phallotoxin to identify F-actin filaments under the fluorescent microscope. All experiments were repeated three times. D EMT biomarkers, including E-cadherin, MMP2, and Vimentin were detected by western blotting 48 h after transfection. E, F Transwell migration (E1) and invasion (F1) assay were performed on GC cells. Quantification of migration and invasion capabilities of GC cells are shown (E2, F2). ***P < 0.01, ****P < 0.001. G The wound healing assay was used to detect cell motility of AGS and MKN-74 cells following transfection. The migration index is shown in the right panel. **P < 0.01, ***P < 0.01, ****P < 0.001. Scale bars, 25 μm in (B) and 10 μm in (C).