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. 2023 May 24;14(5):341. doi: 10.1038/s41419-023-05862-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The expression of EMT biomarkers, including E-cadherin, MMP2, and Vimentin was detected by western blotting 48 h following transfection. B, C GC cells were transfected and subjected to Transwell migration and invasion assay. Quantification of migration and invasion capabilities of GC cells are shown in the right panel. ****P < 0.001. D The mobility of transfected AGS cells was detected by the wound healing assay and the migration index is shown in the right panel. **P < 0.05, ***P < 0.01, ****P < 0.001. E Representative images of metastatic tumours in the lungs from mice in the Control + Vector, PAXIP1-AS1 + Vector, and PAXIP1-AS1 + PABPC1 groups are shown, respectively (n = 3 in each group). F The number of metastatic tumours in the lung was counted. ***P < 0.001, ****P < 0.001. G, H IHC staining of E-cadherin and MMP2 expressions. I, J Representative images (I) and qualification (J) of hepatic metastatic tumours in the indicated groups (n = 3). ***P < 0.01. Scale bar, 100 μm in (G) and (H).