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. 2023 May 24;14(5):341. doi: 10.1038/s41419-023-05862-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A Scr siRNA and HOXD9 siRNAs were transfected into GC cells. The expression of HOXD9, PABPC1 and PAK1 was detected in GC cells using western blotting analysis. B, C Expression levels of HOXD9, PABPC1, PAK1, and PAXIP1-AS1 in 15 paired normal and GC tissue specimens were detected by western blotting (B) or qPCR (C) and normalised to GAPDH. N, normal; T, cancer. Student’s t-test; *P > 0.05; ***P < 0.01 and ****P < 0.001, normal vs. cancer. D–I Pearson correlation analysis was used to identify expression correlations between HOXD9 and PAXIP1-AS1 (D), HOXD9 and PABPC1 (E), HOXD9 and PAK1 (F), PAXIP1-AS1 and PABPC1 (G), PAXIP1-AS1 and PAK1 (H), and PABPC1 and PAK1 (I) using the expression data above. J The expression of indicated genes in normal and GC tissues was evaluated using IHC and ISH. Scale bar, 100 μm. K The hypothetical model depicts the roles of HOXD9, PAXIP1-AS1, PABPC1, and PAK1 in GC cells.