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. 2022 Nov 14;48(7):1000–1010. doi: 10.1038/s41386-022-01502-2

Fig. 6. Inhibition of PKA and expression of GSK3βS9A prevent the lithium rescue in AnkG knockdown neurons.

Fig. 6

A Confocal images of GFP from cultured rat neurons transfected for three days with control or AnkG RNAi with or without HA-GSK3βS9A and treated with or without lithium chloride and Rp-8-Br-cAMPS, scale = 10 µm. B–D Scatter plots showing spine density, spine length, and spine head width (20 neurons from 2 independent experiments, 1-way ANOVA with Dunnett’s post-test, **p < 0.01, #p < 0.0001, ±SEM). E Representative traces of cultured rat neurons expressing control or AnkG RNAi, +/- HA-GSK3βS9A and treated with or without lithium chloride and Rp-8-Br-cAMPS, scale = 100 µm. F–H Sholl analysis graph for total, basal, and apical dendrites (41–46 neurons from 3 independent experiments, 2-way ANOVA with Dunnett’s post-test row repeated measures, *p < 0.05, #p < 0.0001, ±SEM).