Table 2.
Results obtained in samples, including the two cytological examinations performed presented in counted cells per field, the colony forming units (CFU) per plate in both Sabouraud’s glucose agar (SGA) and modified Dixon agar (mDA), and qPCR quantification values
| Sample | Dog | Cytological examination (cells/field) | Culture CFU | qPCR | ||
|---|---|---|---|---|---|---|
| Tape strip | Heat-fixed slide | SGA | mDA | ITS nº of copies | ||
| Samples from dogs with pododermatitis | ||||||
| 1 | Dog 1 | 1.3 | 1.1 | 79 | 66 | 2.6 × 107 |
| 2 | Dog 2 | 0.2 | 0 | 1 | 0 | 1.4 × 106 |
| 3 | Dog 3 | 12.6 | 0.4 | 218 | 1 | 1.2 × 107 |
| 4 | Dog 4 | 1.4 | 0.5 | 76 | 14 | 7.5 × 106 |
| 5 | Dog 5 | 6.2 | 2.2 | 2 | 9 | 2.1 × 108 |
| 6 | Dog 5 | 11.4 | 0.6 | 120 | 311 | 8.1 × 107 |
| 7 | Dog 6 | 8.4 | 0.2 | 4 | 0 | 1.7 × 107 |
| 8 | Dog 7 | 45.9 | 0.7 | 468 | 153 | 2.7 × 108 |
| 9 | Dog 8 | 6.8 | 0.3 | 172 | 91 | 1.3 × 106 |
| 10 | Dog 8 | 1.3 | 0.3 | 10 | 17 | 1.6 × 106 |
| 11 | Dog 9 | 4.7 | 0.2 | 38 | 15 | 1.6 × 108 |
| 12 | Dog 9 | 2.3 | 0 | 3 | 1 | 1.9 × 107 |
| 13 | Dog 10 | 4.7 | 1.3 | 59 | 24 | 2.8 × 108 |
| 17 | Dog 13 | 3.2 | 0 | 2 | 1 | 2.2 × 106 |
| 18 | Dog 13 | 0.1 | 0 | 5 | 1 | 6.5 × 106 |
| Samples from healthy dogs | ||||||
| 14 | Dog 11 | 0 | 0 | 0 | 0 | 4.9 × 106 |
| 15 | Dog 11 | 0 | 0 | 0 | 0 | 1.1 × 105 |
| 16 | Dog 12 | 0 | 0 | 0 | 0 | 1.2 × 106 |
| Samples after antifungal treatment | ||||||
| 19 | Dog 3 | 0.2 | 0 | 0 | 0 | 1.3 × 107 |
| 20 | Dog 7 | 0 | 0 | 0 | 0 | 3.7 × 105 |
| 21 | Dog 8 | 0 | 0 | 0 | 0 | 6.5 × 105 |
| 22 | Dog 8 | 0 | 0 | 0 | 0 | 1.7 × 105 |
aIn bold, samples analysed by NGS