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. 2022 Dec 22;47(2):885–900. doi: 10.1007/s11259-022-10052-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Zeta potential (ZP) of bovine uterine fluid extracellular vesicles (UF-EV). UF-EV were acquired from live cows (LC) and slaughtered cows (SC) at follicular (FP) and luteal phases (LP). The EV were isolated with combined tangential flow filtration (TFF) and size exclusion chromatography (SEC) methods. EV samples labelled with CellMask™ Green Plasma Membrane Stain (CMG) in fluorescent mode (FL-NP) were measured at minimum brightness threshold value of 25, while the rest were measured with 30. The significant differences of ZP between measurement groups are marked with asterisk (*) symbol. EV only: EV sample measured in scatter mode, FL-NP: EV samples labelled with CMG dye measured in fluorescent mode, total-NP: EV samples labelled with CMG dye measured in scatter mode