. 2023 Apr 20;50(6):4945–4953. doi: 10.1007/s11033-023-08401-9

Table 1.

The primers used for PCR amplification of the tested carbapenemase-encoding genes

Gene	Primer	Primer sequence (5ʹ ◊ 3ʹ)	Expected PCR product size (bp)	T_a (°C)	References
bla _NDM	P_f	GGTTTGGCGATCTGGTTTTC	621	50	[41, 42]
bla _NDM	P_r	CGGAATGGCTCATCACGAT	621	50	[41, 42]
bla _KPC	P_f	TGTCACTGTATCGCCGTC	1011	50	[40]
bla _KPC	P_r	CTCAGTGCTCTACAGAAAACC	1011	50	[40]
bla _OXA-48	P_f	GCGTGGTTAAGGATGAACAC	438	50	[40, 42]
bla _OXA-48	P_r	CATCAAGTTCAACCCAACCG	438	50	[40, 42]
bla _VIM	P_f	TCTACATGACCGCGTCTGTC	748	50	[43]
bla _VIM	P_r	TGTGCTTTGACAACGTTCGC	748	50	[43]
bla _IMP	P_f	CTACCGCAGCAGAGTCTTTG	587	50	[44]
bla _IMP	P_r	AACCAGTTTTGCCTTACCAT	587	50	[44]

Notes: bla_NDM, bla_KPC, bla_OXA-48,bla_VIM, and bla_IMP genes code for NDM, KPC, OXA-48-like, VIM, and IMP carbapenemases, respectively. Abbreviations: T_a, annealing temperature; P_f, forward primer; P_r, reverse primer