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. 2023 May 25;61(5):2202022. doi: 10.1183/13993003.02022-2022

FIGURE 4.

FIGURE 4

Chronic treatment with MUC-031 reduces airway mucus plugging and inflammation in adult βENaC-Tg mice with chronic muco-obstructive lung disease. Adult βENaC-Tg mice and wild-type (WT) littermates were treated with MUC-031 or vehicle alone by intratracheal instillation twice daily for 2 weeks. a) Representative airway histology of βENaC-Tg and WT mice after chronic treatment. Sections were stained with Alcian blue–periodic acid–Schiff (AB-PAS) to determine the presence of intraluminal and intraepithelial mucus. Scale bars=100 µm. b–d) Quantification of b) total, c) intraluminal and d) intraepithelial mucus content determined by measuring the volume density of AB-PAS positive material in proximal main axial airways (n=15–22 per group). e, f) Effects of e) chronic treatment with MUC-031 on inflammatory cell counts and f) concentrations of keratinocyte chemoattractant (KC), tumour necrosis factor (TNF)-α and interleukin (IL)-13 in bronchoalveolar lavage (n=10 per group). *: p<0.05, ***: p<0.001, ****: p<0.0001 compared with vehicle-treated WT mice; #: p<0.05, ##: p<0.01 compared with vehicle-treated βENaC-Tg mice.