Figure 5.

In vivo comparison of intensity- and MFLI-FRET dynamic imaging. (A) Maps at selected time points of intensity-based $f_{D}E$ in the liver and the urinary bladder of a mouse injected with Tf A:D 80:40 μg (total time points: 210). (B) Corresponding intensity-based $f_{D}E$ time trace. (C) Maps at selected time points of lifetime-based $f_{D}E$ in the liver and the urinary bladder of a mouse injected with Tf A:D 80:40 μg (total time points: 127). (D) Corresponding lifetime-based $f_{D}E$ time trace. For (B) and (D), solid lines mark the average value, and the shaded areas indicate the standard deviation across all pixels within each organ ROI.