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Purification of XMAS-binding nuclear proteins
| Fraction | Concn (μg/μl) | Total protein (μg) | Binding activitya (BSU) | Sp act (BSU/μg) | Purification (fold) |
|---|---|---|---|---|---|
| Crude nuclear extract | 2 | 13,000 | 5,500 | 0.42 | 1 |
| Ammonium sulfate precipitation | 16.5 | 3,300 | 2,600 | 0.79 | 1.9 |
| Heparin-Sepharose | 1.9 | 570 | 370 | 0.65 | 1.5 |
| X-HIV DNA affinity | 0.01 | 0.5 | 200 | 400 | 950 |
A band shift unit (BSU) is the amount of probe retarded in the presence of 2 μg of Jurkat nuclear extract and a large excess of probe.
