Figure 3.

CTSK promotes the proliferation of HCC cells, related to Figure S1

(A) Western blotting revealed the inhibitory effect of Balicatib on CTSK protein levels in Huh7 and HepG2 cells.

(B) Representative images of the EdU experiment. Scale bar: 200 μm.

(C) Statistical analysis of EdU experiments performed in HuH7 and HepG2 cells after treatment with Balicatib.

(D) Results of the CCK-8 assay.

(E) Western blotting revealed the overexpression efficiency of CTSK.

(F) Representative images of the EdU experiment. Scale bar: 200 μm.

(G) Statistical analysis of EdU experiments performed in Huh7 and HepG2 cells after the overexpression of CTSK. (H) Results of the CCK-8 assay. All quantitative data are means ± SEM from three independent experiments (n = 3). The value of first day in CCK-8 assay was labeled “1”. ∗p < 0.05, ∗∗p < 0.01, as evaluated by paired two-tailed Student’s t test.