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. 2023 May 25;24:138. doi: 10.1186/s12931-023-02441-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Vaping exposure significantly changes T cell populations in spleen and blood of exposed animals. Animals were exposed to FA, PGVG or PGVG + phytol as described. At the end of the 8 week exposure, exposed animals were injected IV with anti-CD45 5 min prior to sacrifice, and spleens A–C and blood D–F were processed, stained, and analyzed by flow cytometry for number of CD45 + CD3 + CD4 + T cells (A, D), CD45 + CD3 + CD4 + CD25hi T cells (B, E), and CD45 + CD3 + CD4 + CD44hiCD62Llo T cells (C, F). T-test p values between 2 conditions indicated by line 2 experiments, p value for one way ANOVA of all 3 conditions shown below. n = 3 each for total of 6