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Schematic of the study design including the workflow. Fecal samples were collected from 20 healthy adults and each fecal sample was divided into five aliquots. One aliquot was supplied as it was (D0), and another was frozen and stored at −80°C for 28 days (F28). Another three aliquots were stored in Metabolokeeper® at 30°C for 7 (D7), 14 (D14), and 28 (D28) days. The microbiota and metabolites in each specimen were analyzed.
