TABLE 2.
mAb neutralization of H5 escape viruses.
| Virus a | mAb concentration for 50% neutralization (μg/mL) b | ||||
|---|---|---|---|---|---|
| 1F3 | 3D6 | 4C12 | 5C2 | 6D9 | |
| A/dk/Bangladesh | <0.3 | <0.3 | 1.25 | 5 | <0.3 |
| 1F3v (R189K) | 20 | 0.16 | >320 | 0.6 | 80 |
| 4C12v (D154N) | >80 | 80 | >80 | 1.25 | >80 |
| 5C2v (K48E) | 1.25 | <0.3 | 5 | >80 | 1.25 |
| 6D9v (K152T) | >80 | 5 | >80 | 1.25 | >80 |
| 1C2 | 1E1 | 3H4 | 5H3 | 8D1 | |
| A/Sichuan | 20 | 20 | 5 | <0.3 | 1.25 |
| 1C2v (N154D) | >80 | >80 | >80 | >80 | >80 |
| 1E1v (E126G) | >80 | >80 | >80 | >80 | >80 |
| 3H4v (N154D) | >80 | >80 | >80 | >80 | >80 |
| 5H3v (A156T) | >80 | >80 | >80 | >80 | >80 |
Abbreviation: mAb, monoclonal antibody.
a
Each virus was titrated and diluted to approximately 500 pfu/mL and incubated with mAb concentrations from 80 to 0.3 μg/mL for incubation with mAb in a plaque‐reduction neutralization experiment.
b
All mAbs completely neutralized their parent virus at concentrations between 0.3 and 20 μg/mL (gray shading in the table); escape mutants that were neutralized by each mAb are also shown in gray.