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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Nat Neurosci. 2023 Apr 20;26(5):788–797. doi: 10.1038/s41593-023-01306-7

Extended Data Fig. 7 |. Dynamics of CA3 CB subtypes and all CA2 interneuron subtypes around short- and long-duration SWRs.

Extended Data Fig. 7 |

(a) Average Z-scored peri-SWR traces for both short- (0–20th percentile) and long- (80–100th percentile) duration SWRs for CA3 CB/SATB1 + interneurons (n = 8 CB/SATB1+ cells from n = 13 mice). Traces for each condition represent mean ± s.e.m. (b) Average Z-scored peri-SWR traces for both short- (0–20th percentile) and long- (80–100th percentile) duration SWRs for CA3 CB/SATB1− interneurons (n = 13 CB/SATB1− cells from n = 13 mice). Traces for each condition represent mean ± s.e.m. (c) Average value of the difference in activity between long- and short-duration SWRs for CA3 CB+/SATB1+ and CB+/SATB1− subtypes, considered separately for the pre-SWR and post-SWR activity. Neither CB+/SATB1+ nor CB+/SATB1− interneurons responded differently during long-duration compared to short-duration SWRs (two-way, one-sample t-tests against 0 for each subtype and condition: CB+/SATB1+ PRE: 0.028 ± 0.176, p = 0.69; CB+/SATB1+ POST: 0.124 ± 0.251, p = 0.23; CB+/SATB1−PRE: −0.047 ± 0.150, p = 0.30; CB+/SATB1−POST: 0.056 ± 0.239, p = 0.43). Data from 8 CB+/SATB1+ and 13 CB+/SATB1− cells from n = 13 mice. Data reported as mean ± s.d. (d) Average Z-scored peri-SWR traces for both short- (0–20th percentile) and long- (80–100th percentile) duration SWRs for CA2 interneurons of each subtype (n = 46 PVBCs, 46 AACs, 59 SOMs, 19 CCKs, and 22 CBs from n = 13 mice). CB+/SATB1+ and CB+/SATB1− neurons are considered together in the CB subtype. Traces for each subtype and condition represent mean ± s.e.m. (e) Average value of the difference in activity between long- and short-duration SWRs for each CA2 interneuron, considered separately for the pre-SWR and post-SWR activity, and grouped by subtype. PVBCs were significantly more activated after long-duration SWRs, while the other subtypes did not show different dynamics during short-compared to long-duration SWRs. Only significant differences are indicated (two-way, one-sample t-tests against 0 for each subtype with Bonferroni correction for multiple testing: PVBC PRE: −0.044 ± 0.264, p = 1.0; PVBC POST: 0.267 ± 0.464, p = 0.0037; AAC PRE: 0.024 ± 0.250, p = 1.0; AAC POST: −0.053 ± 0.322, p = 1.0; SOM PRE: 0.054 ± 0.389, p = 1.0; SOM POST: 0.042 ± 0.340, p = 1.0; CCK PRE: −0.116 ± 0.252, p = 0.70; CCK POST: −0.036 ± 0.360, p = 1.0; CB PRE: −0.050 ± 0.351, p = 1.0; CB POST: 0.103 ± 0.328, p = 1.0). Data from n = 46 PVBCs, 46 AACs, 59 SOMs, 19 CCKs, and 22 CBs from n = 13 mice. CB+/SATB1+ and CB+/SATB1− neurons are considered together in the CB subtype. Data reported as mean ± s.d. (f) Average Z-scored peri-SWR traces for both short- (0–20th percentile) and long- (80–100th percentile) duration SWRs for CA2 CB+/SATB1+ interneurons (n = 12 CB+/SATB1+ cells from n = 13 mice). Traces for each condition represent mean ± s.e.m. (g) Average Z-scored peri-SWR traces for both short- (0–20th percentile) and long- (80–100th percentile) duration SWRs for CA2 CB/SATB1− interneurons (n = 8 CB+/SATB1− cells from n = 13 mice). Traces for each condition represent mean ± s.e.m. (h) Average value of the difference in activity between long- and short-duration SWRs for CA2 CB+/SATB1+ and CB+/SATB1− subtypes, considered separately for the pre-SWR and post-SWR activity. Neither CB+/SATB1+ nor CB+/SATB1− interneurons responded differently during long-duration compared to short-duration SWRs (two-way, one-sample t-tests against 0 for each subtype and condition: CB+/SATB1+ PRE: −0.034 ± 0.373, p = 0.77; CB+/SATB1+ POST: 0.068 ± 0.263, p = 0.41; CB+/SATB1− PRE: −0.078 ± 0.333, p = 0.56; CB+/SATB1− POST: 0.058 ± 0.374, p = 0.69). Data from 12 CB+/SATB1+ and 8 CB+/SATB1− cells from n = 13 mice. Data reported as mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.