TABLE 4.
Quantification of viral DNA in latently infected murine trigeminal ganglia
| Virus | No. of viral DNA copies/cella (no. of ganglia) | Pb |
|---|---|---|
| HSV-1(F) | 0.84 ± 0.17 (21) | <0.005 |
| R7538(P−/O−) | 0.14 ± 0.03 (26) | |
| R7543(P−/O−)R | 1.10 ± 0.21 (19) | <0.0001 |
a
Quantitation of PCR-amplified HSV-1 TK gene from 100 ng of purified trigeminal ganglion DNA. Values were extrapolated from a standard curve ranging from 0.0001 to 10 viral DNA copies per cell and normalized for DNA content by comparison to the PCR-amplified gene encoding murine β-actin. Values are averages ± standard error.
b
P values for R7538(P−/O−) latent DNA amounts compared with either HSV-1(F) or R7543(P−/O−)R DNA amounts.