Complete DMEM for culturing NIH-3T3 pre-adipocytes:

Reagent	Final concentration	Amount
Dulbecco’s Modified Eagle’s Medium (DMEM) – high glucose	N/A	440 mL
FBS (fetal bovine serum)	10%	50 mL
L-glutamine	1%	5 mL
Penicillin-Streptomycin (10,000 U/mL)	1%	5 mL
Total	N/A	500 mL

Store the medium at 4°C for at maximum 30 days. Heat to 37°C before use.