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. 2023 May 20;34:101696. doi: 10.1016/j.tranon.2023.101696

Fig. 4.

Fig 4

(A–B) Immunofluorescence analysis of BCKDK in A549 cells was performed by fluorescence microscope (original magnification: 200x). (C–D) The BCKDK protein level of A549 BCKDK KD and BCKDK OV stable cell lines was measured using western blotting (n = 3 for each group). (E–F) The BCKDK protein level of H1299 BCKDK KD and BCKDK OV stable cell lines was measured using western blotting (n = 3 for each group). (G–H) Colony formation assay of BCKDK OV and BCKDK KD H1299 cells. (I) CCK-8 assay of BCKDK OV and BCKDK KD H1299 cells. (J–K) The caspase-3 protein level of BCKDK OV and BCKDK KD H1299 cells was measured using western blotting (n = 3 for each group). (L) Apoptosis analysis of BCKDK OV and BCKDK KD H1299 cells. (M) BCAA assay of BCKDK OV and control A549 cells was conducted (n = 3 for each group).