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(A–E) The p-S6, S6, and Rab1A protein levels of BCKDK KD and control A549 cells treated with 7.5 mM leucine at different time gradients were measured using western blotting (n = 3 for each group). (F–J) The p-S6, S6, and Rab1A protein levels of BCKDK KD and control H1299 cells treated with 7.5 mM leucine for different time gradients were measured using western blotting (n = 3 for each group). (K) Apoptosis assay of control H1299 cells treated with 7.5 mM leucine for 1 h.
