Table 1. NGS DNA panel results.

Results with frequency < 5 and/or coverage < 50 were excluded):
Gene	Variant		Amino acid change		Coverage		Allele frequency		Interpretation
BRAF	NM_0004333.4: c.1799T>A		NP_004324.2: p.Val600Glu		4298		49.12 %		relevant
NF1	NM_001042492.2:c.480G>T		NP_001035957.1: p.Arg160Ser		125		5.6 %		unclear relevance
ATRX	NM_000489.4: c.4345A>G		NP_000480.3: p.Lys1449Glu		151		5.3 %		unclear relevance
Regions covered by QIASeq Targeted DNA Panel CDHS-21330Z-424:
Coding regions with splice sites
ATRX		EGFR	NF1	NF2		PTEN		TP53
Hotspot mutations
AKT		BRAF	CTNNB1	FGFR1		FGFR2		H3F3A	HIST1H3B
HIST1H3C		IDH1	IDH2	KRAS		PIK3CA		PIK3R1	TERT (Promoter)