Table 1. Analytical Parameters and Variations Applied for Analysis of Robustness.
parameter | nominal conditions | variations | |||
---|---|---|---|---|---|
A/a | mobile flow rate | 0.3 mL/min | A | 0.5 mL/min | a |
B/b | column temperature | 30 °C | B | 32 °C | b |
C/c | mobile phase (A/B) | 25:75 | C | 30:70 | c |
D/d | Ortho-phosphoric acid concentration in solvent B | 0.01% | D | 0.02% | d |
E/e | wavelength | 271 | E | 260 | e |
371 | 360 |