Table 1. Cell-based screening assay for candidate drugs.

Cell type(s)	Model	Active compounds	References
SPR: surface plasmon resonance; MEFs: NF1-deficient mouse embryonic fibroblasts
MCF-7 Cells/ OR6M1-expressing cell lines	SPR chip immobilized cells. A modified carboxymethyl dextran sensor chip	Anthraquinone, rutin	Choi et al. 2021
DNA-PKcs and OCT4 - expressing HEK-293FT cells/ MK2 and OCT4 expressing NCI-H82 cells	384-well plate. Two-step method. Exogenously expressing proteinsin cancer cells as first step	A cardioglycoside and an isocarbostyril alkaloid, cholesterol-based structures (three compounds)	Mohiuddin et al. 2021
Vero E6 cells	96-well plates. Transfected 2-E plasmids after compounds pre-incubated	34 hits with cell protection activity	Wang et al. 2021
HeLa cells	24-well plates. Treated with compounds followed by labeling of the intracellular Hsp90. Analysis with in-gel fluorescence after cell lysis	157 compounds. Morin	Ueda et al. 2020
COS7 cells	Clean glass. Single-protein tracking in a living cell. Effects are evaluated by diffusion coefficient shift using fluorescence microscopy	Hyperoside for EGFR and ErbB2. DiAB-141 and 2”-O-acetylvitexin for ErbB3	Kim et al. 2021
COS-7 cells	96-well plates. Cells seeded in monolayer and molecules added to the medium	Blebbistatin, para-aminoblebbistatin, para-nitroblebbistatin, jasplakinolide, cytochalasin D, swinholide A	Radnai et al. 2020
HEK293:FLP-InT-REx-BiFC#20 cells	384-well clear-bottom, black-walled microplate. Cells seeded in monolayer and molecules added to the medium	6 compounds. Isocotoin	Xu et al. 2020
Vero E6/A549/Huh7/ LN-18 cells	6-well plate format and 96-well plate. Cells infected viral dilutions. Cells seeded in monolayer and antivirals in infection medium added to cells	Sofosbuvir and ribavirin	Vicenti et al. 2020
HEp-2/ A549/293T cells	96-well plate. Cells seeded in monolayer and molecules and virus added to the medium	Laby A1/A2	Blockus et al. 2020
Human embryonic stem cells/ Fibroblasts from healthy donors and patients	384-well plates. Cells seeded in monolayer and molecules added to the medium	CUDC-907	Kase et al. 2021
HpeG2/ HeLa cells	Culture chamber. 3D electric cell/matrigel-substrate impedance sensing chip. Cells seeded in prechilled matrigel solution and generated 3D structure	Taxol, cisplatin, sorafenib	Pan et al. 2019
HeLa cells	96-well plates and 384-well plates. Cells seeded in monolayer and molecules added to the medium followed with added viral solution	11 compounds. Gemcitabine	Zhang et al. 2017
MEFs	96-well plates. Cells seeded in monolayer and molecules added to the medium	Cantharidin, Nifedipine	Semenova et al. 2017
HeLa cells	384-well clear-bottomed black plates. Cells seeded in monolayer and molecules added to the medium	18 compounds	Hajjar et al. 2017
MDA-MB-231 breast cancer cells/ HUVECs	96-well plates. Cells seeded in monolayer and extracts added to the medium	Cirsimaritin, Cirsium japonicum extract, cirsimaritin	Yeon Park et al. 2017
HepG2 ARE reporter cells	384 well pate format. Cells seeded in monolayer and molecules added to the medium	AZ-628, PHA-767491, SL-327, PAC-1, pifithrin-α, vitamin B12	Liu et al. 2018a
MC3T3-E1-OSE cells	96-well plates. Cells seeded in monolayer and molecules added to the medium	4 compounds. T63	Zhao et al. 2017
ARPE-19 cells	96-well plate. Cells seeded in monolayer and molecules added to the medium	47 compounds	Maruyama et al. 2018
MG-63 cells	96-well plate. Cells seeded in monolayer and molecules added to the medium	42 compounds	Wang et al. 2020a
A549 cells	384-well plates. Cells seeded in monolayer and infected with DENV1-4, and treated immediately with compounds	(S)-29	Kounde et al. 2017