FIG. 7.

Electron micrographs of thin sections of replicate cultures of mock-infected HEL fibroblasts either untreated (A) or treated with dexamethasone (B) or sorbitol (C) or infected with wild-type HSV-1(F) (10 PFU/cell [E]) or d120 (10 PFU/cell [D]). The infected cells were harvested at 18 h after infection. Osmotic shock was induced in cells exposed to 1 M sorbitol for 1 h. The cells were then incubated in DMEM supplemented with 1% calf serum for 5 h before being harvested. As a positive control for programmed cell death, HEL fibroblasts were exposed to 500 μM dexamethasone for 18 h. The cells were fixed with glutaraldehyde, sectioned, and examined in a Siemens 102 electron microscope. The sections were photographed at ×6,000 (A), ×6,000 (B), ×5,000 (C), ×8,000 (D), or ×5,000 (E).