Table 1.
Loci | PCR primers | PCR: thermal cycles: (Annealing temp. in bold) | Reference |
---|---|---|---|
ITS | ITS1/ITS4 | (95 °C: 30 s, 48 °C: 30 s, 72 °C: 1 min) × 35 cycles | White et al. 1990 |
cal | CAL228F/CAL737R | (95 °C: 15 s, 54 °C: 20 s, 72 °C: 1 min) × 35 cycles | Carbone and Kohn 1999 |
his3 | CYLH3F/H3-1b | (95 °C: 30 s, 57 °C: 30 s, 72 °C: 1 min) × 35 cycles | Crous et al. 2004 |
Glass and Donaldson 1995 | |||
tef1 | EF1-728F/EF1-986R | (95 °C: 15 s, 54 °C: 20 s, 72 °C: 1 min) × 35 cycles | Carbone and Kohn 1999 |
tub2 | T1(Bt2a)/Bt2b | (95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles | Glass and Donaldson 1995 |
O’Donnell and Cigelnik 1997 |