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. 2023 Mar 1;95:209–228. doi: 10.3897/mycokeys.95.98969

Table 1.

Loci used in this study with PCR primers and process.

Loci PCR primers PCR: thermal cycles: (Annealing temp. in bold) Reference
ITS ITS1/ITS4 (95 °C: 30 s, 48 °C: 30 s, 72 °C: 1 min) × 35 cycles White et al. 1990
cal CAL228F/CAL737R (95 °C: 15 s, 54 °C: 20 s, 72 °C: 1 min) × 35 cycles Carbone and Kohn 1999
his3 CYLH3F/H3-1b (95 °C: 30 s, 57 °C: 30 s, 72 °C: 1 min) × 35 cycles Crous et al. 2004
Glass and Donaldson 1995
tef1 EF1-728F/EF1-986R (95 °C: 15 s, 54 °C: 20 s, 72 °C: 1 min) × 35 cycles Carbone and Kohn 1999
tub2 T1(Bt2a)/Bt2b (95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles Glass and Donaldson 1995
O’Donnell and Cigelnik 1997