Fig. 3.
The infiltrating monocyte-derived cells exhibit a long-term engraftment in the retina. A Representative flow cytometry plots showing the gating strategy for distinguishing resident macrophages (YFP+) from recruited monocyte-derived counterparts (YFP−) based on YFP expression in retinas of Cx3cr1CreER:R26-YFP mice at various timepoints ranging from 2 to 168 dpi ONC + P3C. Cells were pre-gated as live single CD11b+CD45+ Ly6G-CX3CR1+ F480+ as shown in Fig. 2A. B Compiled flow cytometry data showing the percentage of YFP+ and YFP− cells in the macrophage gate (live single CD11b+CD45+ Ly6G-CX3CR1+ F480+) at different time points after ONC + P3C ranging from 2 to 168 dpi ONC in the retina. Data are shown as mean ± SEM. n = 3–8 biologically independent samples containing 1 retina from 1 mouse. C Cell count of YFP+ resident macrophages (microglia and BAMs) and YFP− recruited MDMs at different time points after ONC + P3C ranging from 2 to 168 dpi ONC in the retina, as measured by flow cytometry. Data are shown as mean ± SEM. Repeated measures one-way ANOVA followed by Tukey’s multiple comparisons test, statistical significance between different time points is indicated using different letters: conditions that share the same letter are not significantly different, while conditions with different letters are significantly different from each other. n = 3–8 biologically independent samples with 1 retina from 1 mouse